• high quality

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  developed for the World Health Organization (WHO)

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  used in WHO international research projects

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  used in 25 centres worldwide

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  extraction RIA not subject to interference problems associated with direct serum/plasma immunoassays

• high performance

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  detection limit 150 pmol/L (47pg/mL)

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  between-assay CV better than 15% in the range 600-4000 pmol/L (188 - 1253 pg/mL)

• convenient

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  all principal reagents are provided

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  uses inexpensive, robust equipment

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  each kit is sufficient for 100 assay tubes

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  shelf life of 1 year at 4-8°C

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  three internal Quality Control samples are provided

For information and prices please contact us.

The assay is an extraction competitive RIA for the quantitative measurement of Levonorgestrel (LNG) in human serum or plasma. The LNG extraction RIA is not subject to the well-documented interference problems associated with direct serum/plasma immunoassays. Levonorgestrel in the sample is extracted with diethyl ether. The extract is dried and redissolved in Assay Buffer. The redissolved extract is assayed for levonorgestrel by 3H-RIA (tritium labelled radioimmunoassay with charcoal separation). The samples and standards are incubated with levonorgestrel tritium tracer and antibody. Separation of free levonorgestrel from antibody-bound levonorgestrel is achieved by means of a charcoal separation reagent. The assay has five main stages.

• Levonorgestrel in the sample (200 µL) is extracted with diethyl ether. The extract is dried and redissolved in Assay Buffer. Levonorgestrel standards are prepared in Assay Buffer.
• Anti-levonorgestrel antibody and tritium labelled levonorgestrel tracer are incubated with standards and samples for 18-24 hours at 4°C.
• A charcoal separation reagent is added, and incubated for 30-35 minutes at 4°C., followed by centrifugation at 4°C for 5 minutes.
• After centrifugation, the supernatants are transferred to tubes containing scintillation cocktail.
• The tubes are then placed in a Beta (liquid scintillation) Counter. The radioactive count of each tube can be measured and the results calculated using a data processing program.

LNG RIA Standard LNG RIA Antiserum LNG RIA Tritium Label LNG RIA Charcoal Reagent

LNG RIA Dextran Reagent LNG RIA Gelatin Reagent LNG RIA Internal QC Samples (Low, Medium andHigh) Instruction booklet

Distilled/deionised water, Scintillation Cocktail, Diethyl ether, Sodium dihydrogen phosphate, Disodium hydrogen phosphate, Sodium chloride, Thimerosal.

Glass test tubes, Pipettes, Vortex mixer, Magnetic stirrer, Centrifuge, Water bath, and Beta (liquid scintillation) counter.