• high quality

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  developed for the World Health Organization (WHO)

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  used in WHO international research projects

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  used in 10 countries world-wide

• high performance

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  detection limit 1 nmol/L

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  within-assay CVs generally less than 10%

• low cost

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  all reagents are provided - only test tubes and water are required

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  uses inexpensive, robust equipment

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  each kit is sufficient for 100 assay tubes

• convenient

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  magnetic separation

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  shelf life of 1 year at 4-8°C

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  two internal Quality Control samples are provided

For information and prices please contact us.

The assay is a competitive EIA for the quantitative measurement of Pregnanediol-3a-Glucuronide (PDG) in human urine. Urine samples to be measured are diluted in Assay Buffer. Pregnanediol-3a-Glucuronide in the diluted sample then equilibrates with an anti-Pregnanediol-3a-Glucuronide rabbit antibody and alkaline phosphatase labelled Pregnanediol-3a-Glucuronide. Separation of free Pregnanediol-3a-Glucuronide from antibody-bound Pregnanediol-3a-Glucuronide is achieved by means of a magnetic anti-rabbit separation reagent. A further incubation with substrate produces colour in inverse proportion to the amount of Pregnanediol-3a-Glucuronide present. The assay has four main stages:

• Urine samples and Pregnanediol-3a-Glucuronide standards are prepared by dilution in Assay Buffer.
• Alkaline phosphatase labelled Pregnanediol-3a-Glucuronide, anti-Pregnanediol-3a-Glucuronide antibody and magnetic anti-rabbit separation reagent are incubated with diluted sample (250 mL) for 2 hours at 37°C.
• A coloured enzyme substrate is incubated with the particles for 1 hour at 37°C. The presence of alkaline phosphatase causes a colour change from yellow to pink. The reaction is terminated by addition of Stop Buffer.
• The tubes are then placed in the spectrophotometer or colorimeter. The optical density (at 550nm) of each tube can be measured and the results calculated using a data processing program.

PDG EIA Standard PDG EIA Antiserum PDG EIA Enzyme Label PDG EIA Separation Reagent PDG EIA Assay Buffer PDG EIA Wash Buffer

EIA Substrate Reagent EIA Substrate Buffer EIA Stop Buffer PDG EIA Internal QC Samples (Low and High) Instruction booklet

Test tubes, pipettes, multivortex mixer, magnetic separator, 37°C water-bath and a colorimeter or spectrophometer.