• high quality

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  developed for the World Health Organization (WHO)

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  used in WHO international research projects

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  used in 25 countries worldwide

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  performs well in national and international EQA Schemes

• high performance

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  detection limit 2 nmol/L

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  within-assay CV better than 15% in range 2 - 50 nmol/L

• low cost

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  all reagents are provided - only test tubes and water are required

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  uses inexpensive, robust equipment

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  each kit is sufficient for 100 assay tubes

• convenient

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  direct serum assay (no pre-extraction of samples required)

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  magnetic separation

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  shelf life of 1 year at 4-8°C

For information and prices please contact us.

The assay is a direct competitive EIA for the quantitative measurement of Progesterone in human serum or plasma. Progesterone in the sample is displaced from serum binding proteins by a blocking reagent and bound by a monoclonal anti-Progesterone antibody. The complex is isolated by adsorbtion onto a magnetic anti-mouse IgG separation reagent, and unoccupied anti-Progesterone sites are bound by alkaline phosphatase labelled Progesterone tracer. The assay has four main stages:

• Anti-Progesterone antibody, blocking reagent and magnetic anti-mouse IgG are incubated with sample (50 µL) for 2 hours at 37°C followed by a magnetic wash step.
• The magnetic particles are incubated with alkaline phophatase labelled Progesterone tracer for 15 minutes at 37°C followed by 2 magnetic wash steps.
• A coloured enzyme substrate is incubated with the particles for 1 hour at 37°C. The presence of alkaline phosphatase causes a colour change from yellow to pink. The reaction is terminated by addition of Stop Buffer.
• The tubes are then placed in the spectrophotometer or colorimeter. The optical density (at 550nm) of each tube can be measured and the results calculated using a data processing program.

Incubation times	2hrs + 15 mins + 1 hr
Standard Range	0-100 nmol/L 0-31.5 ng/mL
Normal Ranges	Female day 20-24 >14.3 |nmol/L Male 1.3-3.6 |nmol/L
Detection Limit	2   nmol/L
Assay Reproducibility	Within-batch ±1SD nmol/L %CV 9.3±0.6 6.2 21.4±1.8 8.5 37.7±3.7 9.9 40.0±2.8 6.9
Assay Reproducibility	Between-batch ±1SD nmol/L %CV 9.5±1.4 14.2 15.4±1.8 12.0 22.7±2.0 8.7 38.5±3.1 8.2

Progesterone EIA Standards Progesterone EIA Antiserum Progesterone EIA Blocking Reagent Progesterone EIA Separation Reagent Progesterone EIA Enzyme Label Progesterone EIA Assay Buffer Progesterone EIA Wash Buffers

EIA Substrate Reagent EIA Substrate Buffer EIA Stop Buffer EIA Internal QC Sample Instruction booklet

Test tubes, pipettes, multivortex mixer, magnetic separator, 37°C water bath and a colorimeter or spectrophotometer.