• high quality

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  developed for the World Health Organization (WHO)

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  used in WHO international research projects

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  used in 25 countries worldwide

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  performs well in national and international EQA Schemes

• high performance

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  detection limit 10 mIU/L WHO IRP 84/500

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  between-assay CV better than 10% in range 20 - 3000 mIU/L

• low cost

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  all reagents are provided - only test tubes and water are required

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  uses inexpensive, robust equipment

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  each kit is sufficient for 100 assay tubes

• convenient

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  magnetic separation

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  2 hours total incubation time

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  shelf life of 1 year at 4-8°C

For information and prices please contact us.

The assay is an immunometric ('sandwich') EIA for the quantitative measurement of Prolactin in human serum or plasma. Prolactin in the sample is bound by 2 anti-Prolactin antibodies directed at different epitopes. One antibody is attached to magnetic particles and the other is labelled with alkaline phosphatase. The assay has four main stages:

• Magnetic anti-Prolactin is incubated with sample (100 µL) for 15 minutes at 37°C followed by a magnetic wash step.
• The magnetic particles are incubated with alkaline phophatase labelled anti-Prolactin for 1 hour at 37°C followed by 2 magnetic wash steps.
• A coloured enzyme substrate is incubated with the particles for 30 minutes at 37°C. The presence of alkaline phosphatase causes a colour change from yellow to pink. The reaction is terminated by addition of Stop Buffer.
• The tubes are then placed in the spectrophotometer or colorimeter. The optical density (at 550nm or 492nm) of each tube can be measured and the results calculated using a data processing program.

Incubation times	15 mins + 1 hr + 30 mins
Standard Range	0-2500 mIU/L Standardised against WHO IRP 84/500
Normal Ranges	Female 78-455 mIU/L Male 77-208 mIU/L
Detection Limit	10   mIU/L
Assay Reproducibility	Within-batch ±1SD mIU/L %CV 68±3 4.3 203±4 2.0 371±14 3.8 517±11 2.1
Assay Reproducibility	Between-batch ±1SD mIU/L %CV 103±3 3.0 244±23 9.4 372±9 2.5 556±55 9.8

Prolactin EIA Standards - see (*) Prolactin EIA Magnetic Antibody Prolactin EIA Enzyme Labelled Antibody Prolactin EIA Assay Buffer Prolactin EIA Wash Buffer

EIA Substrate Reagent EIA Substrate Buffer EIA Stop Buffer EIA Internal QC Sample Instruction booklet

(*) calibrated against WHO IRP 84/500

Test tubes, pipettes, multivortex mixer, magnetic separator, 37°C water-bath and a colorimeter or spectrophometer.