• high quality

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  developed for the World Health Organization (WHO)

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  used in WHO international research projects

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  used in 10 countries world-wide

• high performance

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  detection limit 0.1 nmol/L

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  within-assay CVs generally less than 10%

• low cost

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  all reagents are provided - only test tubes and water are required

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  uses inexpensive, robust equipment

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  Each kit is sufficient for 100 assay tubes

• convenient

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  magnetic separation

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  shelf life of 1 year at 4-8°C

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  two internal Quality Control samples are provided

For information and prices please contact us.

The assay is a competitive EIA for the quantitative measurement of Estrone-3-Glucuronide (E1G) in human urine. Urine samples to be measured are diluted in Assay Buffer. Estrone-3-Glucuronide in the diluted sample then equilibrates with an anti-Estrone-3-Glucuronide rabbit antibody and alkaline phosphatase labelled Estrone-3-Glucuronide. Separation of free Estrone-3-Glucuronide from antibody-bound Estrone-3-Glucuronide is achieved by means of a magnetic anti-rabbit separation reagent. A further incubation with substrate produces colour in inverse proportion to the amount of Estrone-3-Glucuronide present. The assay has four main stages:

• Urine samples and Estrone-3-Glucuronide standards are prepared by dilution in Assay Buffer.
• Alkaline phosphatase labelled Estrone-3-Glucuronide, anti-Estrone-3-Glucuronide antibody and magnetic anti-rabbit separation reagent are incubated with diluted sample (500 mL) for 2 hours at 37°C.
• A coloured enzyme substrate is incubated with the particles for 1 hour at 37°C. The presence of alkaline phosphatase causes a colour change from yellow to pink. The reaction is terminated by addition of Stop Buffer.
• The tubes are then placed in the spectrophotometer or colorimeter. The optical density (at 550nm) of each tube can be measured and the results calculated using a data processing program.

E1G EIA Standard E1G EIA Antiserum E1G EIA Enzyme Label E1G EIA Separation Reagent E1G EIA Assay Buffer E1G EIA Wash Buffer

EIA Substrate Reagent EIA Substrate Buffer EIA Stop Buffer E1G EIA Internal QC Samples (Low and High) Instruction booklet

Test tubes, pipettes, multivortex mixer, magnetic separator, 37°C water-bath and a colorimeter or spectrophometer.