• high quality

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  developed for the World Health Organization (WHO)

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  used in WHO international research projects

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  used in 10 centres worldwide

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  extraction RIA not subject to interference problems associated with direct serum/plasma immunoassays

• high performance

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  detection limit 190 pmol/L (57pg/mL)

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  between-assay CV better than 10% in the range 500-4000 pmol/L (150-1200 pg/mL)

• convenient

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  all principal reagents are provided

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  uses inexpensive, robust equipment

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  each kit is sufficient for 100 assay tubes

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  shelf life of 1 year at 4-8°C

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  three internal Quality Control samples are provided

For information and prices please contact us.

The assay is an extraction competitive RIA for the quantitative measurement of Norethisterone (NET) in human serum or plasma. The NET extraction RIA is not subject to the well-documented interference problems associated with direct serum/plasma immunoassays. Norethisterone in the sample is extracted with diethyl ether. The extract is dried and redissolved in Assay Buffer. The redissolved extract is assayed for norethisterone by 3H-RIA (tritium labelled radioimmunoassay with charcoal separation). The samples and standards are incubated with norethisterone tritium tracer and antibody. Separation of free norethisterone from antibody-bound norethisterone is achieved by means of a charcoal separation reagent. The assay has five main stages.

• Norethisterone in the sample (100 µL) is extracted with diethyl ether. The extract is dried and redissolved in Assay Buffer. Norethisterone standards are prepared in Assay Buffer.
• Anti-norethisterone antibody and tritium labelled norethisterone tracer are incubated with standards and samples for 18-24 hours at 4°C.
• A charcoal separation reagent is added, and incubated for 30-35 minutes at 4°C., followed by centrifugation at 4°C for 5 minutes.
• After centrifugation, the supernatants are transferred to tubes containing scintillation cocktail.
• The tubes are then placed in a Beta (liquid scintillation) Counter. The radioactive count of each tube can be measured and the results calculated using a data processing program.

NET RIA Standard NET RIA Antiserum NET RIA Tritium Label NET RIA Charcoal Reagent

NET RIA Dextran Reagent NET RIA Gelatin Reagent NET RIA Internal QC Samples (Low, Medium and High) Instruction booklet

Distilled/deionised water, Scintillation Cocktail, Diethyl ether, Sodium dihydrogen phosphate, Disodium hydrogen phosphate, Sodium chloride, Thimerosal.

Glass test tubes, Pipettes, Vortex mixer, Magnetic stirrer, Centrifuge, Water bath, and Beta (liquid scintillation) counter.