• high performance

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  detection limit 0.3 nmol/L (0.1 ng/ml)

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  within-assay CV less than 10% between 0.5 - 60 nmol/L (0.2 - 17 ng/mL)

• low cost

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  all reagents are provided - only test tubes and water are required

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  uses inexpensive, robust equipment

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  Each kit is sufficient for 100 assay tubes

• convenient

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  direct serum assay (no pre-extraction of samples required)

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  magnetic separation

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  shelf life of 9 months at 4-8°C

For information and prices please contact us.

The assay is a direct competitive EIA for the quantitative measurement of Testosterone in human serum or plasma. A specific agent displaces Testosterone from binding proteins, making it available for antibody binding. Testosterone then equilibrates with an anti-Testosterone antibody linked to fluorescein isothiocyanate (FITC) and alkaline phosphatase labelled Testosterone. An anti-FITC antibody bound to magnetic particles then separates the testosterone/testosterone label-antibody complex from unbound components. A further incubation with substrate produces colour in inverse proportion to the amount of Testosterone present. The assay has four main stages:

• Displacing agent, alkaline phosphatase labelled testosterone and anti-testosterone antibody conjugated to FITC are incubated with sample (50 µL) for 30 minutes at 37°C.
• Anti-FITC antibody coupled to magnetic particles is added and incubated for 5 minutes at 37°C. Anti-testosterone antibody is then isolated by means of a magnetic wash step.
• A coloured enzyme substrate is incubated with the particles for 30 minutes at 37°C. The presence of alkaline phosphatase causes a colour change from yellow to pink. The reaction is terminated by addition of Stop Solution.
• The tubes are then placed in the spectrophotometer or colorimeter. The optical density (at 550nm) of each tube can be measured and the results calculated using a data processing program.

See diagram popup.

Incubation times	30 mins + 5 mins + 30 mins
Standard Range	0-59 nmol/L 0-17 ng/mL
Normal Ranges	Male 8.71-36.47 nmol/L Female <0.35-3.33 nmol/L
Detection Limit	<0.3   nmol/L
Assay Reproducibility	Within-batch ±1SD nmol/L %CV 2.2±0.07 3.1 14.3±0.7 4.6 34.6±1.8 5.3
Assay Reproducibility	Between-batch ±1SD nmol/L %CV 8.0±0.4 5.6 15.8±1.5 9.3 30.6±2.0 6.5

Testosterone EIA Standards Testosterone EIA Antiserum Testosterone EIA Separation Reagent Testosterone EIA Enzyme Label Testosterone EIA Wash Concentrate

EIA Substrate Reagent EIA Substrate Buffer EIA Stop Buffer Testosterone EIA Internal QC Sample Instruction booklet

Test tubes, pipettes, multivortex mixer, magnetic separator, 37°C water-bath and a colorimeter or spectrophometer.